Abberior Instruments STED/RESOLFT

Super resolution microscopy

Location

CIID, BSL2, EG, Room 045

Spatial Light Modulator (SLM) 2D/3D STED (resolution down to 25nm in 2D and 75nm in 3D)

Adaptive Illumination (RESCue, DyMIN)

Environment Control

Fluorescence Lifetime Imaging Microscopy (FLIM)

Lasers:

405nm, cw, 50mW

485nm, pulsed, 1mW@40MHz

561nm, pulsed, 200uW@40MHz

640nm, pulsed, 1mW@40MHz

775nm STED laser, pulsed, 3W@40MHz

Detection:

3 APDs (spectral detection)

Objectives:

Olympus 40x, UPLFLN, 1.3NA Oil, 0.2mm WD

Olympus 60x, UPLSAPO 1.2NA water, 0.28mm WD

Olympus 100x, UPLSAPO 1.4NA Oil, 0.13mm WD

Book the system here

Click here for materials and methods template